fulltext.study @t Gmail

Size characterization of inclusion bodies by sedimentation field-flow fractionation

Paper ID Volume ID Publish Year Pages File Format Full-Text
24730 43533 2008 7 PDF Available
Title
Size characterization of inclusion bodies by sedimentation field-flow fractionation
Abstract

Sedimentation field-flow fractionation (sedFFF) was evaluated to characterize the size of Δ(4–23)TEM-β-lactamase inclusion bodies (IBs) overexpressed in fed-batch cultivations of Escherichia coli. Heterologous Δ(4–23)TEM-β-lactamase protein formed different sizes of IBs, depending upon the induction conditions. In the early phases of recombinant protein expression, induced with low concentrations of IPTG (isopropyl-β-d-thiogalactoside), IB masses were larger than expected and showed heterogeneous size distributions. During cultivation, IB sizes showed a Gaussian distribution and reached a broad range by the end of the fed-batch cultivations. The obtained result proved the aptitude of sedFFF to rapidly assess the size distribution of IBs in a culture.

Keywords
SedFFF, Sedimentation field-flow fractionation; IB, inclusion body; IPTG, isopropyl-β-d-thiogalactopyranoside; BDM, biomass dry matter; SDS–PAGE, sodium dodecyl sulphate polyacryl gel electrophoresis; Trp, tryptophanSedimentation field-flow fractionation;
First Page Preview
Size characterization of inclusion bodies by sedimentation field-flow fractionation
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 138, Issues 3–4, 25 November 2008, Pages 67–73
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering