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E2F-1 overexpression increases viable cell density in batch cultures of Chinese hamster ovary cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
24735 43533 2008 4 PDF Available
Title
E2F-1 overexpression increases viable cell density in batch cultures of Chinese hamster ovary cells
Abstract

The cell density is an inherent constraint in commercial mammalian cell cultures. Here, we describe a cell engineering strategy utilizing the overexpression of the E2F-1 cell cycle transcription factor in CHO DG44 cells that produce a monoclonal antibody in serum-free, suspension culture. Stable pools and cell lines expressing E2F-1 were isolated that attained viable cell densities 20% higher than control cell lines and continued proliferation for an additional day in batch culture. There were no significant changes in antibody production, apoptosis, and cell cycle compared to control cells, nor were the growth effects evident in fed-batch conditions. Overall, E2F-1 overexpression postponed entry into stationary phase in mammalian cells, but perhaps novel E2F-1 variants or combination cell cycle engineering strategies will be necessary to realize significant growth benefits in commercial applications.

Keywords
Cell cycle; Cell cycle engineering; E2F; E2F-1; Mammalian cell culture; Cell engineering
First Page Preview
E2F-1 overexpression increases viable cell density in batch cultures of Chinese hamster ovary cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 138, Issues 3–4, 25 November 2008, Pages 103–106
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering