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Ear mesenchymal stem cells: An efficient adult multipotent cell population fit for rapid and scalable expansion

Paper ID Volume ID Publish Year Pages File Format Full-Text
24741 43534 2009 9 PDF Available
Title
Ear mesenchymal stem cells: An efficient adult multipotent cell population fit for rapid and scalable expansion
Abstract

Bone marrow mesenchymal stem cells (BM-MSCs) have the potential to be used for tissue engineering. Nevertheless, they exhibit a low growth rate that limits their availability. In this work we use an alternative model of MSCs from the outer ear (ear mesenchymal stem cells, E-MSCs). These cells bear the characteristics of progenitor cells because of their ability to be differentiated into the three lineages of chondrocytes, osteocytes and adipocytes. This model cell population had a threefold higher cell growth rate compared to BM-MSCs. This allowed rapid testing of the scalability in microcarrier culture using bead-to-bead transfer and also enabled their expansion in a 1-l bioreactor. The cells were able to maintain their potential for differentiation into the above three lineages. Therefore, E-MSCs appear to be an attractive model for assessing a number of bioengineering parameters that may affect the behavior of adult stem cells in culture.

Keywords
Mesenchymal stem cells; Microcarrier; Stirred culture; Growth rate; Differentiation potential
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Ear mesenchymal stem cells: An efficient adult multipotent cell population fit for rapid and scalable expansion
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 139, Issue 4, 23 February 2009, Pages 291–299
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us