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A novel hybrid dual-channel catalytic-biological sensor system for assessment of fruit quality

Paper ID Volume ID Publish Year Pages File Format Full-Text
24744 43534 2009 4 PDF Available
Title
A novel hybrid dual-channel catalytic-biological sensor system for assessment of fruit quality
Abstract

The release of volatile ethylene and acetaldehyde characterizes the metabolic state and quality of fruit. We have designed and implemented a hybrid dual-channel catalytic-biological sensor system, which is able to quantify both volatiles in situ. This sensor system consists of a mammalian cell line engineered for constitutive expression of an Aspergillus nidulans-derived biosensor which triggers quantitative reporter gene expression in the presence of volatile acetaldehyde. Ethylene, oxidized to acetaldehyde using a Wacker-based process, can be quantified by the same transgenic sensor cell line. Differential profiling of reporter gene transcription by the sensor system revealed the relative concentrations of both volatile metabolites and enabled correct assessment of fruit quality as shown for fresh, old and rotten apples. Functional combination of catalytic processes with biosensor technology is able to precisely capture the metabolic state of food and may foster novel insight into biochemical food quality assessment as well as the design of synthetic control circuits detecting and preventing food spoilage.

Keywords
Acetaldehyde; Biosensor; Ethylene; Fruit quality; Synthetic biology; Wacker
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A novel hybrid dual-channel catalytic-biological sensor system for assessment of fruit quality
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 139, Issue 4, 23 February 2009, Pages 314–317
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us