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Antitumor effect of F-PBFβ-TrCP-induced targeted PTTG1 degradation in HeLa cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
24749 43535 2009 6 PDF Available
Title
Antitumor effect of F-PBFβ-TrCP-induced targeted PTTG1 degradation in HeLa cells
Abstract

Pituitary tumor-transforming gene 1 (PTTG1), a proto-oncogene, is associated with tumor formation, proliferation and invasiveness. F-PBFβ-TrCP, a fusion protein, was produced by replacing the WD40-repeat of F-box protein β-TrCP with the PTTG1-binding factor (PBF) for targeted degradation of PTTG1. To evaluate the function of F-PBFβ-TrCP, PTTG1-EGFP fusion protein was constructed. Our results showed that F-PBFβ-TrCP can both degrade exogenous PTTG1-EGFP fusion protein in COS-7 cells and endogenous PTTG1 protein in HeLa cells and the targeted PTTG1 knock down resulted in bFGF mRNA level down-regulation and inhibition of proliferation and clonogenicity in HeLa cells. In conclusion, targeted degradation of PTTG1 by F-PBFβ-TrCP has antitumor activity in vitro in HeLa cells. These results suggest that F-PBFβ-TrCP could be used for cancer treatment by targeted degradation of PTTG1.

Keywords
PTTG1; PTTG1-binding factor; F-box; Targeted degradation; Growth inhibition
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Antitumor effect of F-PBFβ-TrCP-induced targeted PTTG1 degradation in HeLa cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 139, Issue 1, 1 January 2009, Pages 6–11
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us