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Construction of multifunctional proteins for tissue engineering: Epidermal growth factor with collagen binding and cell adhesive activities

Paper ID Volume ID Publish Year Pages File Format Full-Text
24751 43535 2009 7 PDF Available
Title
Construction of multifunctional proteins for tissue engineering: Epidermal growth factor with collagen binding and cell adhesive activities
Abstract

The development of different techniques based on natural and polymeric scaffolds are useful for the design of different biomimetic materials. These approaches, however, require supplementary steps for the chemical or physical modification of the biomaterial. To avoid such steps, in the present study, we constructed a new multifunctional protein that can be easily immobilized onto hydrophobic surfaces, and at the same time helps enhance specific cell adhesion and proliferation onto collagen substrates. A collagen binding domain was fused to a previously constructed protein, which had an epidermal growth factor fused to a hydrophobic peptide that allows for cell adhesion. The new fusion protein, designated fnCBD-ERE–EGF is produced in Escherichia coli, and its abilities to bind to collagen and promote cell proliferation were investigated. fnCBD-ERE–EGF was shown to keep both collagen binding and cell growth-promoting activities comparable to those of the corresponding unfused proteins. The results obtained in this study also suggest the use of a fnCBD-ERE–EGF as an alternative for the design of multifunctional ECM-bound growth factor based materials.

Keywords
Epidermal growth factor; Cell adhesion; Fusion protein; Hydrophobic structure; Collagen binding
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Construction of multifunctional proteins for tissue engineering: Epidermal growth factor with collagen binding and cell adhesive activities
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 139, Issue 1, 1 January 2009, Pages 19–25
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us