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High-titer production of monomeric hydroxyvalerates from levulinic acid in Pseudomonas putida

Paper ID Volume ID Publish Year Pages File Format Full-Text
24757 43535 2009 7 PDF Available
Title
High-titer production of monomeric hydroxyvalerates from levulinic acid in Pseudomonas putida
Abstract

Hydroxyacids represent an important class of compounds that see application in the production of polyesters, biodegradable plastics and antibiotics, and that serve as useful chiral synthetic building blocks for other fine chemicals and pharmaceuticals. An economical, high-titer method for the production of 4-hydroxyvalerate (4HV) and 3-hydroxyvalerate (3HV) from the inexpensive and renewable carbon source levulinic acid was developed. These hydroxyvalerates were produced by periodically feeding levulinate to Pseudomonas putida KT2440 expressing a recombinant thioesterase II (tesB) gene from Escherichia coli K12. The titer of 4HV in shake flask culture reached 13.9 ± 1.2 g L−1 from P. putida tesB+ cultured at 32 °C in LB medium periodically supplemented with glucose and levulinate. The highest 3HV titer obtained was 5.3 ± 0.1 g L−1 in M9 minimal medium supplemented with glucose and levulinate.

Keywords
3-Hydroxyvalerate; 4-Hydroxyvalerate; tesB; Pseudomonas putida
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High-titer production of monomeric hydroxyvalerates from levulinic acid in Pseudomonas putida
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 139, Issue 1, 1 January 2009, Pages 61–67
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us