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A simple and effective system for foreign gene expression in plants via root absorption of agrobacterial suspension

Paper ID Volume ID Publish Year Pages File Format Full-Text
24784 43536 2008 5 PDF Available
Title
A simple and effective system for foreign gene expression in plants via root absorption of agrobacterial suspension
Abstract

Due to the laborious and scale-up limitation we have developed a simple system named “root absorption” to express foreign proteins in plants successfully. It has been shown that GFP was expressed in tobacco plants by root absorbing the Agrobacterium suspension containing TMV-based P35S-30B-GFP vector. Various factors influencing the gene expression were studied including Agrobacterium cell density, seedling age, plant materials and inoculation conditions. This system has the special advantages as simple and convenient work process, ease to scale-up and higher level of expression than leaf infiltration. Interestingly, GFP was expressed at 24 h post-absorption. We assume that the root absorption system will facilitate the large-scale production of the recombinant pharmaceutical proteins in plants by means of transient expression.

Keywords
Root absorption system; Plant bioreactor; Agroinoculation; Tobacco mosaic virus (TMV)
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A simple and effective system for foreign gene expression in plants via root absorption of agrobacterial suspension
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 134, Issues 3–4, 30 April 2008, Pages 320–324
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us