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Proliferation of NS0 cells in protein-free medium: The role of cell-derived proteins, known growth factors and cellular receptors

Paper ID Volume ID Publish Year Pages File Format Full-Text
24790 43537 2009 7 PDF Available
Title
Proliferation of NS0 cells in protein-free medium: The role of cell-derived proteins, known growth factors and cellular receptors
Abstract

NS0 cells proliferate without external supply of growth factors in protein-free media. We hypothesize that the cells produce their own factors to support proliferation. Understanding the mechanisms behind this autocrine regulation of proliferation may open for the novel approaches to improve animal cell processes. The following proteins were identified in NS0 conditioned medium (CM): cyclophilin A, cyclophilin B (CypB), cystatin C, D-dopachrome tautomerase, IL-25, isopentenyl-diphosphate delta-isomerase, macrophage migration inhibitory factor (MIF), β2-microglobulin, Niemann pick type C2, secretory leukocyte protease inhibitor, thioredoxin-1, TNF-α, tumour protein translationally controlled 1 and ubiquitin. Further, cDNA microarray analysis indicated that the genes for IL-11, TNF receptor 6, TGF-β receptor 1 and the IFN-γ receptor were transcribed. CypB, IFN-α/β/γ, IL-11, IL-25, MIF, TGF-β and TNF-α as well as the known growth factors EGF, IGF-I/II, IL-6, leukaemia inhibitory factor and oncostatin M (OSM) were excluded as involved in autocrine regulation of NS0 cell proliferation. The receptors for TGF-β, IGF and OSM are however present in NS0 cell membranes since TGF-β1 caused cell death, and IGF-I/II and OSM improved cell growth. Even though no ligand was found, the receptor subunit gp130, active in signal transduction of the IL-6 like proteins, was shown to be essential for NS0 cells as demonstrated by siRNA gene silencing.

Keywords
NS0 myeloma cells; Autocrine growth factors; Regulation of proliferation; Conditioned medium; gp130
First Page Preview
Proliferation of NS0 cells in protein-free medium: The role of cell-derived proteins, known growth factors and cellular receptors
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 141, Issues 3–4, 20 May 2009, Pages 123–129
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering