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Genotyping by allele-specific l-DNA-tagged PCR

Paper ID Volume ID Publish Year Pages File Format Full-Text
24822 43539 2008 4 PDF Available
Title
Genotyping by allele-specific l-DNA-tagged PCR
Abstract

We have developed a novel method for typing single-nucleotide polymorphisms (SNPs) that can be applicable to rapid screening. The method involves the fusion of two PCR techniques, allele-specific PCR (AS-PCR) and l-DNA-tagged PCR (LT-PCR), which enables us to label PCR products with sequence-defined tags of mirror-image DNA (l-DNA). PCR products were applied without any purification or denaturation steps to gold surfaces where complementary single-stranded l-DNA was immobilized, and the products were detected with surface plasmon resonance (SPR) imaging. We were able to clearly discriminate 3 genotypes at position 2677 of the MDR1 gene (G/G-homozygote, G/T-heterozygote, and T/T-homozygote) by comparing SPR difference images.

Keywords
Single nucleotide polymorphism (SNP) typing; Allele-specific PCR; l-DNA; Surface plasmon resonance (SPR) imaging
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 135, Issue 2, 1 June 2008, Pages 157–160
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us