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A chemical lipid modification of recombinant preS antigen to study the mechanism of HBV attachment to the host cell

Paper ID Volume ID Publish Year Pages File Format Full-Text
24834 43540 2008 6 PDF Available
Title
A chemical lipid modification of recombinant preS antigen to study the mechanism of HBV attachment to the host cell
Abstract

Surface antigen preS of Hepatitis B virus plays fundamental roles in mediating receptor recognition and virus internalization. Myristoylation at N-terminal Gly2 residue of preS is essential for viral attachment and infectivity. A number of myristoylated proteins have been shown to undergo a conformational change (myristoyl switch) that alters their affinity to cell membrane. However, there is little knowledge about what effect this fatty acylation contributes in virus–host cell interaction. Here we demonstrated a new method for lipid modification of recombinant preS protein at N-terminal residue 2 with alkylating chemicals. Modified preS was able to inhibit HBV penetrating into HepG2 cells with an increased efficiency compared to unmodified form. Flow cytometric analysis indicated that lipid modification enhanced the binding affinity of preS to hepatocytes, but not resulting from hydrophobic interaction. CD analysis further revealed a conformational change of modified preS in the presence of membrane mimetics. These findings imply that the conformation transition induced by fatty acylation is important for efficient attachment of virus to cell receptors, and this method of chemical lipid modification provides a basis for designing therapeutic inhibitors to Hepatitis B virus.

Keywords
Chemical lipid modification; Hepatitis B virus; preS; Virus attachment; Myristoyl switch
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A chemical lipid modification of recombinant preS antigen to study the mechanism of HBV attachment to the host cell
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 137, Issues 1–4, 10 October 2008, Pages 8–13
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us