fulltext.study @t Gmail

Proteomic analysis of the effect of bile salts on the intestinal and probiotic bacterium Lactobacillus reuteri

Paper ID Volume ID Publish Year Pages File Format Full-Text
24835 43540 2008 6 PDF Available
Title
Proteomic analysis of the effect of bile salts on the intestinal and probiotic bacterium Lactobacillus reuteri
Abstract

Lactobacillus reuteri is a resident of the human and animal intestinal tracts. The ability of L. reuteri to survive passage through the intestinal tract is a key point in its function as a probiotic. In order to examine the nature of bile salt tolerance by L. reuteri, its protein synthesis was analyzed in liquid cultures containing two different bile salt conditions. Significant cell growth inhibition was observed in the presence of 1.2 g/L (higher concentration) bile salts. Two-dimensional gel electrophoresis allowed us to identify 28 proteins spots that were consistently and significantly altered in the presence of bile in the growth medium. Peptide mass fingerprinting was used to identify these 28 proteins, and functional annotation revealed their involvement in carbohydrate metabolism, transcription–translation, nucleotide metabolism, amino acid biosynthesis, pH homeostasis and stress responses, oxidation–reduction reactions, and unknown functions. These findings, which suggest that bile salts induce complex physiological responses in L. reuteri may provide early new insights into the inducible mechanisms underlying the capacity of intestinal L. reuteri to tolerate bile stress.

Keywords
CHAPS, 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate; DTT, dithiothreitol; IEF, isoelectric focusing; IPG, immobilized pH gradient; MALDI–TOF, matrix-assisted laser desorption/ionization–time-of-flight; LAB, lactic acid bacteriaLactobacillus
First Page Preview
Proteomic analysis of the effect of bile salts on the intestinal and probiotic bacterium Lactobacillus reuteri
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 137, Issues 1–4, 10 October 2008, Pages 14–19
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering