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Simultaneous production of endo-β-1,4-xylanase and branched xylooligosaccharides by Thermomyces lanuginosus

Paper ID Volume ID Publish Year Pages File Format Full-Text
24838 43540 2008 10 PDF Available
Title
Simultaneous production of endo-β-1,4-xylanase and branched xylooligosaccharides by Thermomyces lanuginosus
Abstract

When grown on beech-wood glucuronoxylan, two strains of the thermophilic fungus Thermomyces lanuginosius, IMI 84400 and IMI 96213, secreted endo-β-1,4-xylanase of glycoside hydrolase family 11 and simultaneously accumulated an acidic pentasaccharide in the medium. The aldopentaouronic acid was purified and its structure was established by a combination of NMR spectroscopy and enzyme digestion with glycosidases as MeGlcA3Xyl4.Both strains showed limited growth on wheat arabinoxylan as a carbon source. An essential part of the polysaccharide was not utilized, and it was converted to a series of arabinoxylooligosaccharides differing in the degree of polymerization. The structure of the shorter arabinoxylooligosaccharides remaining in the wheat arabinoxylan-spent medium was established using mass spectrometry and digestion with glycosidases. Xylose and linear β-1,4-xylooligosaccharides generated extracellularly during growth on either hardwood or cereal xylan were efficiently taken up by the cells and metabolized intracellularly. The data suggest that due to a lack of extracellular β-xylosidase, α-glucuronidase, and α-l-arabinofuranosidase, the widely used T. lanuginosus strains might become efficient producers of branched xylooligosaccharides from both types of xylans.

Keywords
AnArf, Aspergillus niger α-l-arabinofuranosidase; BspArf, Bifidobacterium sp. α-l-arabinofuranosidase; GH, glycoside hydrolase; MeGlcA, 4-O-methyl-d-glucuronic acid or 4-O-methyl-α-d-glucopyranuronosyl; MeGlcA3Xyl4, β-d-xylopyranosyl-1,4-[2-O-(4-O-methyl-
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Simultaneous production of endo-β-1,4-xylanase and branched xylooligosaccharides by Thermomyces lanuginosus
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 137, Issues 1–4, 10 October 2008, Pages 34–43
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us