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Isolation of two laccase genes from the white-rot fungus Pleurotus eryngii and heterologous expression of the pel3 encoded protein

Paper ID Volume ID Publish Year Pages File Format Full-Text
24843 43541 2008 11 PDF Available
Title
Isolation of two laccase genes from the white-rot fungus Pleurotus eryngii and heterologous expression of the pel3 encoded protein
Abstract

In this paper we report the cloning and nucleotide sequence analysis of two new laccase genes from the white-rot fungus Pleurotus eryngii, named pel3 and pel4. Comparison of the protein sequences deduced from these genes with laccases previously described in P. eryngii indicates that these genes codify for new laccases in this fungus. We described the expression of pel3 gene in two different Aspergillus niger strains. Both the laccase signal peptide and the glucoamylase preprosequence of A. niger were used to target the secretion of the active enzyme. The highest levels of laccase expression were obtained by combining the last construction with an A. niger strain deficient in extracellular proteases secretion. The characterization of catalytic properties of the recombinant enzyme, together with the setting-up of a heterologous expression system for pel3, will provide the basis to study the biotechnological applications of this enzyme.

Keywords
Basidiomycete; Phenol oxidases; Copper protein; Protein sequence; Heterologous expression
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Isolation of two laccase genes from the white-rot fungus Pleurotus eryngii and heterologous expression of the pel3 encoded protein
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 134, Issues 1–2, 20 March 2008, Pages 9–19
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us