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A crosslinked inclusion body process for sialic acid synthesis

Paper ID Volume ID Publish Year Pages File Format Full-Text
24858 43541 2008 8 PDF Available
Title
A crosslinked inclusion body process for sialic acid synthesis
Abstract

The propensity of a recombinant protein produced in bacteria to aggregate has been assumed to be unpredictable, and inclusion bodies have been thought of as wasted cell material. However, a target protein can be purposely driven to inclusion bodies, which demonstrate full cell tolerable activity. Sialic acid aldolase, N-terminally fused with the cellulose-binding module of Clostridium cellulovorans, was almost quantitatively physiologically aggregated into active inclusion bodies. These inclusion bodies were entrapped in alginate beads and crosslinked by glutaraldehyde. The immobilized biocatalyst generated by this crosslinked inclusion bodies (CLIB) technology was used in a repetitive batch protocol for sialic acid production that was monitored on-line by flow calorimetry. The required substrate, N-acetyl-d-mannosamine, was obtained by partially improved alkaline epimerization.

Keywords
Enzyme engineering; Immobilization; N-Acetyl-d-neuraminic acid lyase; Physiological aggregation; Biosensor; GlcNAc epimerization
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A crosslinked inclusion body process for sialic acid synthesis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 134, Issues 1–2, 20 March 2008, Pages 146–153
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us