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Abiotic stress and ABA-inducible Group 4 LEA from Brassica napus plays a key role in salt and drought tolerance

Paper ID Volume ID Publish Year Pages File Format Full-Text
24883 43543 2009 9 PDF Available
Title
Abiotic stress and ABA-inducible Group 4 LEA from Brassica napus plays a key role in salt and drought tolerance
Abstract

Late-embryogenesis abundant (LEA) proteins are a family of hydrophilic proteins that form an integral part of desiccation tolerance of seeds. LEA proteins have been also postulated to play a protective role under different abiotic stresses. Their role in abiotic stress tolerance has been well documented for Group 1, 2 and 3 LEAs among the nine different groups. The present study evaluates the functional role of a Group 4 LEA protein, LEA4-1 from Brassica napus. Expression analysis revealed that abscisic acid, salt, cold and osmotic stresses induce expression of LEA4-1 gene in leaf tissues in Brassica species. Conversely, reproductive tissues such as flowers and developing seeds showed constitutive expression of LEA4, which was up-regulated in flowers under salt stress. For functional evaluation of LEA4-1 with regard to stress tolerance, LEA4-1 cDNA was cloned from B. napus, and overexpressed in both Escherichia coli and transgenic Arabidopsis plants. Overexpression of BnLEA4-1 cDNA in E. coli conferred salt and extreme temperature tolerance to the transformed cells. Furthermore, transgenic Arabidopsis plants overexpressing BnLEA4-1 either under constitutive CaMV35S or abiotic stress inducible RD29A promoter showed enhanced tolerance to salt and drought stresses. These results demonstrate that LEA4-1 plays a crucial role in abiotic stress tolerance during vegetative stage of plant development.

Keywords
Abscisic acid; Abiotic stress; Brassica species; Group 4 LEA; Transgenic Arabidopsis
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Abiotic stress and ABA-inducible Group 4 LEA from Brassica napus plays a key role in salt and drought tolerance
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 139, Issue 2, 15 January 2009, Pages 137–145
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us