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The 3′UTR of HIC mRNA improves the production of recombinant proteins in Chinese hamster ovary cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
24885 43543 2009 4 PDF Available
Title
The 3′UTR of HIC mRNA improves the production of recombinant proteins in Chinese hamster ovary cells
Abstract

Positive transcription elongation factor b (P-TEFb) is an important transcriptional regulator which controls 70–80% of RNA polymerase II transcription. It has been reported that the human I-mfa (inhibitor of MyoD family a) domain-containing protein (HIC) interacts with P-TEFb and that expression of HIC cDNA stimulates P-TEFb-dependent transcription. Interestingly, our recent study shows that transcriptional stimulation by HIC is predominately due to the 3′ untranslated region (3′UTR) of HIC mRNA rather than its coding region. In this report, we investigate the effects of HIC 3′UTR on recombinant protein expression in mammalian cells. In transient transfections, overexpression of HIC 3′UTR stimulates transgene expression in several mammalian cell lines and significantly increases the production of human erythropoietin and interferon-γ in Chinese hamster ovary (CHO) cells. This is the first report that demonstrates the improvement of expression of biopharmaceutical proteins by overexpressing a non-coding 3′UTR in CHO cells.

Keywords
3′UTR, 3′ untranslated region; AC, cardiac actin; Cdk9, cyclin-dependent kinase 9; CHO, Chinese hamster ovary; CMV, cytomegalovirus; CTD, carboxyl-terminal domain; EPO, erythropoietin; HEXIM1, hexamethylene bis-acetamide inducible protein 1; HIC, human I-
First Page Preview
The 3′UTR of HIC mRNA improves the production of recombinant proteins in Chinese hamster ovary cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 139, Issue 2, 15 January 2009, Pages 152–155
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering