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Production of o-diphenols by immobilized mushroom tyrosinase

Paper ID Volume ID Publish Year Pages File Format Full-Text
24887 43543 2009 6 PDF Available
Title
Production of o-diphenols by immobilized mushroom tyrosinase
Abstract

The o-diphenols 4-tert-butyl-catechol, 4-methyl-catechol, 4-methoxy-catechol, 3,4-dihydroxyphenylpropionic acid and 3,4-dihydroxyphenylacetic acid were produced from the corresponding monophenols (4-tert-butyl-phenol, 4-methyl-phenol, 4-methoxy-phenol, p-hydroxyphenylpropionic acid and p-hydroxyphenylacetic acid) using immobilized mushroom tyrosinase from Agaricus bisporus. In all cases the yield was Rdiphenol ≥ 88–96%, which, according to the literature, is the highest yield so far, obtained using tyrosinase. The reaction was carried out in 0.5 M borate buffer pH 9.0 which was used to minimize the diphenolase activity of tyrosinase by complexing the o-diphenols generated. Hydroxylamine and ascorbic acid were also present in the reaction medium, the former being used to reduce mettyrosinase to deoxytyrosinase, closing the catalytic cycle, and the latter to reduce the o-quinone produced to o-diphenol. Inactivation of the tyrosinase by ascorbic acid was also minimized due to the formation of an ascorbic acid–borate complex. Concentrations of the o-diphenolic compounds obtained at several reaction times were determined by gas chromatography–mass spectrometry (GC–MS) and UV–vis spectroscopy. The experimental results are discussed.

Keywords
Tyrosinase; Agaricus bisporus; Immobilization; Cinnamic carbohydrate esters; Tyrosinase extraction; o-Diphenols production
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Production of o-diphenols by immobilized mushroom tyrosinase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 139, Issue 2, 15 January 2009, Pages 163–168
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us