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Mass transfer effects on DNA hybridization in a flow-through microarray

Paper ID Volume ID Publish Year Pages File Format Full-Text
24890 43543 2009 7 PDF Available
Title
Mass transfer effects on DNA hybridization in a flow-through microarray
Abstract

The goal of this study is to assess the influence of mass transfer phenomena on DNA hybridization kinetics in a flow-through, porous microarray for fast molecular testing. We present a scaled mathematical model of coupled convection, diffusion and reaction in porous media, which was used to simulate hybridization kinetics and to analyze the influence of convective transport on the reaction rate. In addition to computer simulations, we also present experimental data of hybridization collected on our microarray system for different flow rates. The results reported in this paper provide for a better understanding of the interaction between reaction and mass transfer processes during flow-through hybridization and suggest criteria for system design and optimization.

Keywords
DNA hybridization; Flow-through microarray; Porous membrane; Mathematical modeling
First Page Preview
Mass transfer effects on DNA hybridization in a flow-through microarray
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 139, Issue 2, 15 January 2009, Pages 179–185
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering