Enzymatic synthesis of poly(hydroxyalkanoates) in ionic liquids
Ring-opening polymerization of five lactones catalyzed by Candida antarctica lipase B in ionic liquids yielded poly(hydroxyalkanoates) of moderate molecular weights up to Mn = 13,000. In the ionic liquid 1-butyl-3-methylimidazolium bis(trifluoromethane)-sulfonimide and with a low weight ratio of enzyme to lactone (1:100) we obtained polymers from β-propiolactone, δ-valerolactone, and ɛ-caprolactone with degrees of polymerization as high as 170, 25, and 85, respectively; oligomers from β-butyrolactone and γ-butyrolactone with degrees of polymerization of 5; and a copolymer of β-propiolactone and β-butyrolactone with a degree of polymerization of 180. Water-immiscible ionic liquids were superior to water-miscible ionic liquids. Reducing the water content of the enzyme improved the degree of polymerization by as much as 50% for β-propiolactone and ɛ-caprolactone.
Journal: Journal of Biotechnology - Volume 132, Issue 3, 1 November 2007, Pages 306–313