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A single mutation in P450BM-3 enhances acyl homoserine lactone: Acyl homoserine substrate binding selectivity nearly 250-fold

Paper ID Volume ID Publish Year Pages File Format Full-Text
24917 43545 2008 3 PDF Available
Title
A single mutation in P450BM-3 enhances acyl homoserine lactone: Acyl homoserine substrate binding selectivity nearly 250-fold
Abstract

Quorum sensing is the process by which bacteria alter gene regulation in response to their population density. The enzymatic inactivation of quorum signals has shown promise for use in genetically modified organisms resistant to pathogens. We recently characterized the ability of a cytochrome P450, P450BM-3, to oxidize the quorum sensing signals known as acyl homoserine lactones. The oxidation of the acyl homoserine lactones reduced their activity as quorum signals. The enzyme also oxidized the inactive lactonolysis products, acyl homoserines. The enzyme showed similar binding affinity for the acyl homoserine lactones and acyl homoserines. The latter reaction may lead to problems when lactonases and the P450-dependent system are used in tandem, as oxidation of the acyl homoserines produced by lactonolysis in vivo may compete with acyl homoserine lactone oxidation by the cytochrome P450. We report here that a single mutation (R47S) in P450BM-3 is capable of increasing the acyl homoserine lactone: acyl homoserine substrate binding selectivity of the enzyme nearly 250-fold, reducing the potential for competition by acyl homoserines and significantly enhancing the potential for use of P450BM-3 as part of a pathogen resistance system in genetically modified crops.

Keywords
Quorum sensing; Cytochrome P450; Acyl homoserine lactone; Monooxygenation; P450BM-3; CYP102A1
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A single mutation in P450BM-3 enhances acyl homoserine lactone: Acyl homoserine substrate binding selectivity nearly 250-fold
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 135, Issue 4, 31 July 2008, Pages 374–376
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us