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In situ analysis of the higher-order genome structure in a single Escherichia coli cell

Paper ID Volume ID Publish Year Pages File Format Full-Text
24921 43546 2008 5 PDF Available
Title
In situ analysis of the higher-order genome structure in a single Escherichia coli cell
Abstract

We developed a novel method for the in situ analysis of the higher-order structure of an individual genome from a single Escherichia coli cell using laser tweezers. Initially, condensed DNA was stably grasped by a laser without any chemical modification and without physical attachment to an artificial object such as micro-plastic beads. Under optical transport, the trapped genome gradually unfolded in solution due to viscous friction. Interestingly, the nucleoid DNA from a log-phase cell is almost fully elongated, whereas in the stationary phase, unfolding of the nucleoid is characterized by step-wise elongation of 1.7–5.1 μm, corresponding to a size of 5–15 kbp, and a few tightly packed domains remain along the DNA chain. This suggests the coexistence of tightly packed and swollen domains in the genome in the stationary phase.

Keywords
Optical tweezers; Higher-order structure; Genome; Giant DNA
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In situ analysis of the higher-order genome structure in a single Escherichia coli cell
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 133, Issue 2, 20 January 2008, Pages 172–176
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us