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Direct preparation of giant proteo-liposomes by in vitro membrane protein synthesis

Paper ID Volume ID Publish Year Pages File Format Full-Text
24924 43546 2008 6 PDF Available
Title
Direct preparation of giant proteo-liposomes by in vitro membrane protein synthesis
Abstract

We investigated the direct constitution of membrane proteins into giant liposomes in cell-free (in vitro) protein synthesis. Giant liposomes were present in a translation reaction cocktail of a wheat germ cell-free protein translation system. Apo cytochrome b5 (b5) and its fusion proteins were synthesized and directly localized in the liposomes. After the translation reaction, the proteo-liposomes were isolated by simplified discontinuous density-gradient centrifugation. Apo cytochrome b5 conjugated dihydrofolate reductase (DHFR) was synthesized in the same procedure and the protein was directly displayed on the liposome surface. b5 acts as a “hydrophobic tag” for recruitment to the liposome surface.

Keywords
Giant liposome; Membrane protein; Cytochrome b5; In vitro protein synthesis; Wheat germ lysate
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Direct preparation of giant proteo-liposomes by in vitro membrane protein synthesis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 133, Issue 2, 20 January 2008, Pages 190–195
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us