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Integrated processing and multiple re-use of immobilised lipase by magnetic separation technology

Paper ID Volume ID Publish Year Pages File Format Full-Text
24949 43547 2007 7 PDF Available
Title
Integrated processing and multiple re-use of immobilised lipase by magnetic separation technology
Abstract

High-gradient magnetic separation based processing is demonstrated for the semi-continuous multicycle re-use of a lipase immobilised on magnetic microparticles. The lipase of Candida antarctica A-type (CALA) was immobilised on polyvinyl alcohol coated magnetic particles (1–2 μm diameter) with epoxy functionalisation. The immobilised CALA was used to hydrolyse a model oil-water 2-phase-system composed of a phosphate buffer with tributyrin at up to 3 l scale. The immobilised enzyme was subsequently recovered in a magnetic filter using high-gradient magnetic separation and reapplied in repeated cycles of hydrolysis and recovery. Two different temperatures of 30 and 50 °C, tributyrin concentrations (0.12 and 35 g l−1) and reaction times were tested. In each case the reaction was followed by pH titration using NaOH, as well as by HPLC analysis. Consecutive cycles were conducted for each reaction condition and in total the immobilised CALA was subjected to 20 recovery and re-use cycles, after which ∼14% of the initial specific activity still remained.

Keywords
Enzyme; Immobilisation; Candida antarctica lipase type-A; CALA; 2-Phase-system; HGMS; Recovery
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Integrated processing and multiple re-use of immobilised lipase by magnetic separation technology
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 132, Issue 2, 31 October 2007, Pages 202–208
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us