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Expressed sequence tag analysis of marine fungus Schizochytrium producing docosahexaenoic acid

Paper ID Volume ID Publish Year Pages File Format Full-Text
24954 43548 2008 8 PDF Available
Title
Expressed sequence tag analysis of marine fungus Schizochytrium producing docosahexaenoic acid
Abstract

To investigate docosahexaenoic acid (DHA, C22:6n − 3) biosynthesis pathway in marine fungus Schizochytrium sp. FJU-512, a cDNA library of the fungus was constructed and analyzed. The titers of primary library were up to 5.0 × 106. A total of 4005 ESTs were assembled into 1947 unigenes. Sequences annotation and function analysis were carried out by using Blast, GO and KEGG programs. Compared with other eukaryote genomes, Schizochytrium sp. FJU-512 ESTs shared at least 26.6% genes with Arabidopsis thaliana (E ≤ 10−10). The cDNA (Contig46, assembled by EH401977 and EH404532) and EH40321 were found to encode serine/threonine protein phosphatase type 1 and cell division control protein 2 which were involved in successive binary cell division. Notably, the key enzymes involved in biosynthesis of fatty acid via polyketide synthases (PKS) such as beta-ketoacyl synthase, beta-ketoacyl reductase, hydroxyacyl dehydrogenase, enoyl-CoA hydratase/isomerase, and enoyl reductase were found in the cDNA library. The results indicated that DHA synthesis in Schizochytrium sp. FJU-512 had undergone PKS pathway.

Keywords
Polyunsaturated fatty acid; Docosahexaenoic acid; Schizochytrium; cDNA library; Polyketide synthases
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Expressed sequence tag analysis of marine fungus Schizochytrium producing docosahexaenoic acid
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 138, Issues 1–2, 6 November 2008, Pages 9–16
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us