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Design and validation of a dynamic flow perfusion bioreactor for use with compliant tissue engineering scaffolds

Paper ID Volume ID Publish Year Pages File Format Full-Text
24970 43549 2008 7 PDF Available
Title
Design and validation of a dynamic flow perfusion bioreactor for use with compliant tissue engineering scaffolds
Abstract

In tissue engineering, flow perfusion bioreactors can be used to enhance nutrient diffusion while mechanically stimulating cells to increase matrix production. The goal of this study was to design and validate a dynamic flow perfusion bioreactor for use with compliant scaffolds. Using a non-permanent staining technique, scaffold perfusion was verified for flow rates of 0.1–2.0 mL/min. Flow analysis revealed that steady, pulsatile and oscillatory flow profiles were effectively transferred from the pump to the scaffold. Compared to static culture, bioreactor culture of osteoblast-seeded collagen-GAG scaffolds led to a 27–34% decrease in cell number but stimulated an 800–1200% increase in the production of prostaglandin E2, an early-stage bone formation marker. This validated flow perfusion bioreactor provides the basis for optimisation of bioreactor culture in tissue engineering applications.

Keywords
Bone tissue engineering; Cartilage tissue engineering; Bioreactor culture; Fluid flow; Mechanical stimulation
First Page Preview
Design and validation of a dynamic flow perfusion bioreactor for use with compliant tissue engineering scaffolds
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 133, Issue 4, 29 February 2008, Pages 490–496
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering