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Construction of gene expression systems in insect cell lines using promoters from the silkworm, Bombyx mori

Paper ID Volume ID Publish Year Pages File Format Full-Text
24974 43550 2008 9 PDF Available
Title
Construction of gene expression systems in insect cell lines using promoters from the silkworm, Bombyx mori
Abstract

The promoter regions of the Bombyx mori HSC70-4 and B. mori TCTP genes characterized previously were used for the construction of a series of constitutive gene expression systems active in cultured cells. The relative abilities of these promoters were evaluated by comparing those of a silkworm actin A3 (BmActin3) promoter, which is used widely as the first choice. A series of constitutive expression systems constructed were assayed for the transcription efficiency by connecting four reporter cDNAs, firefly luciferase, 3GFP, Ds-Red, and β-galactosidase gene using the Gateway LR reaction. The insertion of an intron enhancer into the site between the TCTP promoter and gene increased the transcription of the BmTCTP promoter by 10-fold. The insertion of the IE-1 gene and HR3 enhancer to the all three promoters were found to increase the transcription up to 560 times.

Keywords
Constitutive expression system; Transcription efficiency; Reporter cDNAs; Gateway LR reaction; Enhancer
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Construction of gene expression systems in insect cell lines using promoters from the silkworm, Bombyx mori
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 133, Issue 1, 1 January 2008, Pages 9–17
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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