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Insertion of nuclear factor-κB binding sequence into plasmid DNA for increased transgene expression in colon carcinoma cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
24977 43550 2008 6 PDF Available
Title
Insertion of nuclear factor-κB binding sequence into plasmid DNA for increased transgene expression in colon carcinoma cells
Abstract

To increase plasmid DNA (pDNA)-based transgene expression, 5, 10 or 20 repeats of nuclear factor κB (NF-κB) binding sequences were inserted upstream of the cytomegalovirus (CMV) promoter region of a conventional pDNA encoding firefly luciferase (pCMV-Luc) to obtain pCMV-κB5-Luc, pCMV-κB10-Luc and pCMV-κB20-Luc. Murine carcinoma colon 26 cells, in which NF-κB was constitutively activated, were co-transfected with a firefly luciferase-expressing pDNA and a renilla luciferase-expressing pDNA having no NF-κB binding sequences using cationic liposomes. The expression efficiency of pCMV-κB(n)-Luc was evaluated using the ratio of the luciferase activities. Increasing numbers of NF-κB binding sequences significantly increased transgene expression. The expression was increased by NF-κB activators and the effects were marked with pDNA having many NF-κB binding sequences. These results indicate that insertion of NF-κB binding sequences into pDNA is an effective approach to increase transgene expression in cancer cells in which NF-κB is activated.

Keywords
pDNA, plasmid DNA; NF-κB, nuclear factor κB; NPC, nuclear pore complex; ROS, reactive oxygen species; CMV, cytomegalovirus; PMS, phenazine methosulfateGene transfer; Transcription factor; Nuclear factor-κB; Reactive oxygen species; Colon carcinoma cell; N
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Insertion of nuclear factor-κB binding sequence into plasmid DNA for increased transgene expression in colon carcinoma cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 133, Issue 1, 1 January 2008, Pages 36–41
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us