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Novel thermophilic and thermostable lipolytic enzymes from a Thailand hot spring metagenomic library

Paper ID Volume ID Publish Year Pages File Format Full-Text
24978 43550 2008 8 PDF Available
Title
Novel thermophilic and thermostable lipolytic enzymes from a Thailand hot spring metagenomic library
Abstract

Functional screening for lipolytic enzymes from a metagenomic library (origin: Jae Sawn hot spring, Thailand) resulted in isolation of a novel patatin-like phospholipase (PLP) and an esterase (Est1). PLP contained four conserved domains similar to other patatin-like proteins with lipid acyl hydrolase activity. Likewise, sequence alignment analysis revealed that Est1 can be classified as a family V bacterial lipolytic enzyme.Both PLP and Est1 were expressed heterologously as soluble proteins in E. coli and exhibited more than 50% of their maximal activities at alkaline pH, of 7–9 and 8–10, respectively. In addition, both enzymes retained more than 50% of maximal activity in the temperature range of 50–75 °C, with optimal activity at 70 °C and were stable at 70 °C for at least 120 min.Both PLP and Est1 exhibited high Vmax toward p-nitrophenyl butyrate. The enzymes had activity toward both short-chain (C4 and C5) and long chain (C14 and C16) fatty acid esters. The isolated enzymes, are therefore, different from other known patatin-like phospholipases and esterases, which usually show no activity for substrates longer than C10. We suggest that PLP and EstA enzymes are novel and have a; b potential use in industrial applications.

Keywords
Lipolytic enzyme; Metagenomic library; Patatin-like phospholipase; Esterase
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Novel thermophilic and thermostable lipolytic enzymes from a Thailand hot spring metagenomic library
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 133, Issue 1, 1 January 2008, Pages 42–49
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us