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Effects of acid concentration and solvent choice on enzymatic acrylation by Candida antarctica lipase B

Paper ID Volume ID Publish Year Pages File Format Full-Text
24990 43550 2008 7 PDF Available
Title
Effects of acid concentration and solvent choice on enzymatic acrylation by Candida antarctica lipase B
Abstract

Lipase-mediated acrylation is an attractive alternative to more traditional chemical processes, since it provides specific catalysis under mild conditions. A detailed study of the effects of solvent choice and substrate concentrations on the acrylation of octanol by Candida antarctica lipase B (Novozym® 435) is presented. Acrylic acid was found to have a pronounced inhibitory effect. Partial neutralisation of the acid substrate by addition of an organo-soluble base markedly altered the activity profile, indicating the inhibitory mechanism to be related to acid-base interactions. The concentration of acrylic acid to be employed was found to be important in the choice of an appropriate solvent. At low acrylic acid concentrations, the highest rates and conversions were obtained using hydrophobic solvents, whereas at higher acrylic acid concentrations more polar solvents were advantageous.

Keywords
Lipase; Acrylic acid; Acrylate synthesis; Protonation effects
First Page Preview
Effects of acid concentration and solvent choice on enzymatic acrylation by Candida antarctica lipase B
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 133, Issue 1, 1 January 2008, Pages 127–133
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering