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At-line determination of octanoic acid in cultivation broth-An electronic tongue (ET) feasibility study

Paper ID Volume ID Publish Year Pages File Format Full-Text
24994 43550 2008 8 PDF Available
Title
At-line determination of octanoic acid in cultivation broth-An electronic tongue (ET) feasibility study
Abstract

Production monitoring of “natural” 2-heptanone from octanoic acid in an industrial fed-batch cultivation based on Penicillium roqueforti requires development of a method for determination of octanoic acid dissolved in the water phase. An electronic tongue array using six non-specific potentiometric sensors with solid inner contact, and a pH electrode, has been introduced by spiking octanoic acid to a substrate obtained from four different cultivations, representing variations in the relevant industrial matrix. Multivariate calibration was performed on acid concentrations spanning 0.65–20 mmol l−1. Excluding the lowest concentration a global Partial Least Square regression model with a predicted versus measured correlation of 0.98 and a relative root mean square error of prediction of 5.1% (ln units) (RPD = 5.5) signifies a highly acceptable prediction facility. This model was further tested by subjecting it to undiluted as well as diluted samples obtained from a cultivation process in which octanoic acid was catabolized; this led to acceptable prediction errors within the same range as for the global model. It is concluded that the ET sensor array can be applied for determination of octanoic acid in cultivation systems of the general P. roqueforti type.

Keywords
ET, electronic tongue; PDA, potato dextrose agar; PLS, partial least square; RPD, ratio of standard error of performance to standard deviation; RMSEP, root mean square error of prediction; TSB, tryptic soy brothElectronic tongue; Potentiometric sensors; P
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At-line determination of octanoic acid in cultivation broth-An electronic tongue (ET) feasibility study
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 133, Issue 1, 1 January 2008, Pages 162–169
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us