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Effect of oligosaccharide elicitors on bacitracin A production and evidence of transcriptional level control

Paper ID Volume ID Publish Year Pages File Format Full-Text
24998 43551 2007 7 PDF Available
Title
Effect of oligosaccharide elicitors on bacitracin A production and evidence of transcriptional level control
Abstract

The role of oligosaccharides as biotic elicitors for the enhanced production of antibiotics and enzymes has been established in recent years. These findings could have significant promise for the pharmaceutical and biotechnology industries. Before the elicitors could be used at large-scale, reproducible production and high levels of enhancement are essential. In order to ensure the robustness of elicitation and exploit the process an understanding of this phenomenon at the molecular level is required. Bacitracin produced by Bacillus licheniformis is widely used as an animal feed additive and antibiotic against Gram-positive bacteria. In this work we report, the effect of single and multiple additions of different elicitors to B. licheniformis cultures for the production of bacitracin A. The results obtained showed that single addition of oligoguluronate to the cultures enhanced the levels of bacitracin A by 23.3%. Moreover, multiple elicitor addition of oligoguluronate and mannan oligosaccharide further enhanced the production of bacitracin A by 13.2% and 36.5% compared to single elicitor addition and control cultures, respectively. In addition, for the first time, it was found that elicitor supplementation of cultures increased the transcription level of the bacitracin biosynthetic genes bacABC, indicating transcriptional level control of the elicitor.

Keywords
Bacitracin; Bacitracin synthetase; Gene copy number; Real-time PCR; Elicitation; Bacillus licheniformis
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Effect of oligosaccharide elicitors on bacitracin A production and evidence of transcriptional level control
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 131, Issue 4, 30 September 2007, Pages 397–403
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us