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Multiple display of catalytic modules on a protein scaffold: Nano-fabrication of enzyme particles

Paper ID Volume ID Publish Year Pages File Format Full-Text
25002 43551 2007 7 PDF Available
Title
Multiple display of catalytic modules on a protein scaffold: Nano-fabrication of enzyme particles
Abstract

Self assembly is a prerequisite for fabricating nanoscale structures. Here we present a new fusion protein based on the stress-responsive homo-oligomeric protein, SP1. This ring-shaped protein is a highly stable homododecamer, which can be potentially utilized to self-assemble different modules and enzymes in a predicted and oriented manner. For that purpose, a cohesin module (a component of the bacterial cellulosome) was selected, its gene fused in-frame to SP1, and the fusion protein was expressed in Escherichia coli. The cohesin module, specialized to incorporate different enzymes through specific recognition of a dockerin modular counterpart, is used to display new moieties on the SP1 scaffold. The SP1 scaffold displayed 12 active cohesin modules and specific binding to a dockerin-fused cellulase enzyme from Thermobifida fusca. Moreover, we found a significant increase in specific activity of the scaffold-displayed enzymes.

Keywords
SP1, stable protein 1; Coh, cohesin module; Doc, dockerin module; Coh-SP1, cohesin, genetically fused to SP1; Doc-cellulase, dockerin, genetically fused to N-terminus of cellulase; Doc-l-cellulase, dockerin, genetically fused to N-terminus of cellulase vi
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Multiple display of catalytic modules on a protein scaffold: Nano-fabrication of enzyme particles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 131, Issue 4, 30 September 2007, Pages 433–439
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us