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l-Methionine sulfoximine as a novel selection agent for genetic transformation of orchids

Paper ID Volume ID Publish Year Pages File Format Full-Text
25006 43551 2007 7 PDF Available
Title
l-Methionine sulfoximine as a novel selection agent for genetic transformation of orchids
Abstract

A key challenge in molecular breeding of orchids is the creation of efficient and reproducible gene transformation systems. In this study, we report a new transformation method utilizing l-methionine sulfoximine (MSO) as a novel agent for selection of transgenic Dendrobium hybrids D. Madame Thong-In and D. Chao Praya Smile with the bialaphos resistance (bar) gene as a selectable marker. Gene transformation was performed by biolistic bombardment with a 4-day recovery period on MSO-free medium and two selection stages on media with increasing amounts of selection agent, using concentrations of 5 and 10 μM MSO for D. Madame Thong-In, and 0.5 and 2 μM MSO for D. Chao Praya Smile. Independent transgenic orchid lines were obtained and the presence of the transgene was confirmed by PCR and Southern blot analysis. Because of substantial time and economic savings, the new transformation system using MSO as a selection agent will facilitate functional studies on orchid genes and genetic engineering of orchids with commercially valuable traits.

Keywords
Orchid transformation; Dendrobium; l-Methionine sulfoximine; Bialaphos resistance; Particle bombardment
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 131, Issue 4, 30 September 2007, Pages 466–472
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us