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Metabolic flux analysis using stoichiometric models for Aspergillus niger: Comparison under glucoamylase-producing and non-producing conditions

Paper ID Volume ID Publish Year Pages File Format Full-Text
25014 43552 2007 13 PDF Available
Title
Metabolic flux analysis using stoichiometric models for Aspergillus niger: Comparison under glucoamylase-producing and non-producing conditions
Abstract

Aspergillus niger AB1.13 cultures with glucoamylase production (with d-glucose as substrate) and without glucoamylase production (with d-xylose as substrate) were characterized by metabolic flux analysis. Two comprehensive metabolic models for d-glucose- as well as for d-xylose-consumption were used to quantify and compare the metabolic fluxes through the central pathways of carbon metabolism at different pH-values. The models consist of the most relevant metabolic pathways for A. niger including glycolysis, pentose-phosphate pathway, citrate cycle, energy metabolism and anaplerotic reactions comprising two intracellular compartments, the cytoplasm and mitochondrion.When d-xylose was used as the sole carbon source, the relative flux of the substrate through the oxidative pentose-phosphate pathway (PPP) via G6P-dehydrogenase was unaffected by the pH-value of the culture medium. About 30% of d-xylose consumed was routed through the oxidative PPP. In contrast, the flux of d-glucose (i.e., under glucoamylase-producing conditions) through the oxidative PPP was remarkably higher and, in addition was significantly affected by the pH-value of the culture medium (40% at pH 5.5, 56% at pH 3.7, respectively). Summarizing, the flux through the PPP under glucoamylase producing conditions was 30–90% higher than for non-producing conditions.

Keywords
2-oxo-Glutmit, 2-oxo-glutarate (mitochondrial); 3-PG, 2-phospho-glyceraldehyde; AcCoA, acetyl-coenzyme A; AICAR, 5-aminoimidazol-4-N-succino-carboxamidribonucleotide; E4P, erythrose-4-phosphate; DHAP, dihydroxyacetonephosphate; DW, dry weight; G3P, glycer
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Metabolic flux analysis using stoichiometric models for Aspergillus niger: Comparison under glucoamylase-producing and non-producing conditions
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 132, Issue 4, 1 December 2007, Pages 405–417
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us