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A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium

Paper ID Volume ID Publish Year Pages File Format Full-Text
25016 43552 2007 5 PDF Available
Title
A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium
Abstract

A sucrose-inducible promoter system (PsacB) from Bacillus megaterium was identified using a secretome approach. It was successfully employed for the extracellular production of the homologous levansucrase SacB (4252.4 U l−1) and the heterologous green fluorescent protein GFP (7.9 mg gCDW−1). Mutational analysis of B. megaterium PsacB allowed the identification of important promoter elements. The sucrose-inducible promoter provides a useful alternative to the established xylose-inducible promoter system (PxylA) for recombinant gene expression in B. megaterium.

Keywords
Bacillus megaterium; Sucrose-inducible promoter; Recombinant protein production
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A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 132, Issue 4, 1 December 2007, Pages 426–430
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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