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Recycling of bacterial biomass in a process of l-threonine production by means of a recombinant strain of Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
25017 43552 2007 7 PDF Available
Title
Recycling of bacterial biomass in a process of l-threonine production by means of a recombinant strain of Escherichia coli
Abstract

The Gram-negative bacterium Escherichia coli B-3996 represents an interesting host organism for the production of the essential amino acid l-threonine. Microbial processes – especially those of aerobic cultivation – lead to the generation of considerable amounts of biomass, thus lowering the product yield. These are the reasons for studying methods for the recycling of biomass from E. coli. It will be shown that it is possible to disintegrate the microbial biomass – preferably by means of high pressure homogenisation followed by a protease treatment of the resulting slurry of debris – in an efficient way and to recycle at least different amounts of the soluble part as cultivation medium component. By studying the growth and product formation of E. coli no adverse effects have been observed.

Keywords
Biomass recycling; Microbial biomass; Lysis; Sustainable production; Escherichia coli B-3996; Threonine production
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Recycling of bacterial biomass in a process of l-threonine production by means of a recombinant strain of Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 132, Issue 4, 1 December 2007, Pages 431–437
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us