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Accumulation of CdS nanoparticles by yeasts in a fed-batch bioprocess

Paper ID Volume ID Publish Year Pages File Format Full-Text
25023 43552 2007 6 PDF Available
Title
Accumulation of CdS nanoparticles by yeasts in a fed-batch bioprocess
Abstract

The yeasts Schizosaccharomyces pombe and Candida glabrata were successfully cultivated in a fed-batch process at cadmium levels up to 100 mg l−1. S. pombe incorporated 20 mg Cd g−1 dry biomass within 24 h. C. glabrata accumulated 8 mg Cd g−1 dry biomass in 24 h. The higher Cd uptake from S. pombe cells correlate with the elevated glucose concentrations during and at the end of the cultivation. Analysis of the cells with energy-filtering transmission electron microscopy-element specific imaging (EFTEM-ESI) revealed that cadmium is not precipitated outside the cells or at the cell wall but evenly distributed inside the cell plasma. As Cd is highly toxic this indicates that Cd is immobilized by an intracellular detoxification mechanism. Size exclusion chromatography showed that Cd is associated to a protein fraction between 25 and 67 kDa which corresponds to the theoretical molecular weight of CdS nanoparticles of 35 kDa coated with phytochelatins. This structure has been proposed in literature.

Keywords
Nanoparticles; Detoxification; Heavy metal; Cadmium; Coating
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 132, Issue 4, 1 December 2007, Pages 481–486
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
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Full-text PDF Download
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