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A transposon-based strategy to scale up myxothiazol production in myxobacterial cell factories

Paper ID Volume ID Publish Year Pages File Format Full-Text
25028 43553 2008 7 PDF Available
Title
A transposon-based strategy to scale up myxothiazol production in myxobacterial cell factories
Abstract

Myxobacteria are proficient producers of biologically active secondary metabolites. However, efforts to exploit these natural products for the development of new therapeutics and agrochemicals are frequently hampered by low production levels. We describe here a transposon-based strategy to identify genes encoding regulators of secondary metabolite biosynthesis in the myxobacterium Angiococcus disciformis An d48, which produces the highly efficient electron transport inhibitor myxothiazol. Extracts from 1200 transposon mutants were analyzed by HPLC, leading to the identification of six mutants in which myxothiazol production was increased by as much as 30-fold. Identifying the sites of integration coupled with sequencing of flanking regions, showed that some of the inactivated genes encode proteins with similarity to known bacterial regulators such as two-component systems and serine–threonine protein kinases. However, other gene products do not resemble any characterized proteins. Taken together, these data show that this transposon-based strategy is a valuable tool to identify regulatory genes of secondary metabolism, including gene loci which cannot be detected using current in silico approaches.

Keywords
Transposon mutagenesis; Secondary metabolites; Regulation of production; Functional annotation; Strain improvement methodology
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A transposon-based strategy to scale up myxothiazol production in myxobacterial cell factories
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 135, Issue 3, 30 June 2008, Pages 255–261
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us