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A quick and reliable method for monitoring gene expression in actinomycetes

Paper ID Volume ID Publish Year Pages File Format Full-Text
25029 43553 2008 4 PDF Available
Title
A quick and reliable method for monitoring gene expression in actinomycetes
Abstract

Amycolatopsis balhimycina DSM5908 is the producer of the vancomycin-type glycopeptide antibiotic balhimycin. Balhimycin production is controlled by transcriptional regulators and depends on different environmental factors. To analyse the regulatory network Ptba, a representative promoter of the balhimycin gene cluster, was fused to the reporter gene egfp and integrated into the genome of A. balhimycina. Balhimycin production, fluorescence intensity and the amount of tba-transcript were determined under different cultivation conditions in microtiter plates. Thus we could demonstrate that these values are directly correlated. This approach facilitates the analysis of a large number of samples in parallel and it provides the opportunity to easily define conditions to increase balhimycin production.

Keywords
Amycolatopsis balhimycina; Balhimycin; egfp; Fluorescence
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A quick and reliable method for monitoring gene expression in actinomycetes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 135, Issue 3, 30 June 2008, Pages 262–265
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us