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Expressing Vitreoscilla hemoglobin in statically cultured Acetobacter xylinum with reduced O2 tension maximizes bacterial cellulose pellicle production

Paper ID Volume ID Publish Year Pages File Format Full-Text
25142 43555 2007 6 PDF Available
Title
Expressing Vitreoscilla hemoglobin in statically cultured Acetobacter xylinum with reduced O2 tension maximizes bacterial cellulose pellicle production
Abstract

Vitreoscilla hemoglobin (VHb) was constitutively expressed in Acetobacter xylinum to enhance bacterial cellulose (BC) production. A pronounced enhancement of BC production in static culture was observed. Reducing O2 tension in gaseous phase of the culture by tightly sealing the culture tube could also enhance BC production by 70%. O2 tension in gaseous phase reduced from 21 to 15% in the sealed and static culture of VHb-expressing A. xylinum after 7 days cultivation, while 7.36 g/l of BC with yield of 0.44 were obtained. BC pellicle production by VHb-expressing A. xylinum was successfully scaled-up in a sealed 4 l disposable zip lock plastic bag with BC yield of 0.38 and concentration of 6.73 g/l.

Keywords
Bacterial cellulose; Acetobacter xylinum; Vitreoscilla hemoglobin; Reduced O2 tension
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Expressing Vitreoscilla hemoglobin in statically cultured Acetobacter xylinum with reduced O2 tension maximizes bacterial cellulose pellicle production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 132, Issue 1, 15 October 2007, Pages 38–43
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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