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Purification and characterisation of an acid phosphatase with phytase activity from Mucor hiemalis Wehmer

Paper ID Volume ID Publish Year Pages File Format Full-Text
25148 43555 2007 6 PDF Available
Title
Purification and characterisation of an acid phosphatase with phytase activity from Mucor hiemalis Wehmer
Abstract

An acid phosphatase with phytase activity, produced by Mucor hiemalis Wehmer, was purified to homogeneity by a combination of anion exchange, gel filtration and hydrophobic interaction chromatography. The monomeric, glycosylated enzyme displayed maximum activity at 55 °C and pH 5.0–5.5. When compared to commercialised products, the enzyme is more thermostable (80 °C, 5 min), displays a broader pH versus activity profile and greater stability under simulated digestive tract conditions. Unlike commercial phytases, the Mucor enzyme should retain some activity in the small intestine as well as in the stomach, facilitating a longer duration of action and hence more extensive substrate hydrolysis. Substrate specificity studies and protein database similarity searching using mass spectrometry-derived sequence data indicate that the enzyme is an acid phosphatase with activity on phytate. Cocktails containing acid phosphatases in combination with true phytases have been shown to promote more extensive phytate degradation than do true phytases alone. This, coupled to the enzyme's functionally relevant physicochemical characteristics, suggests its likely suitability for inclusion in second generation phytase cocktails for application in animal feed.

Keywords
Phytase; Acid phosphatase; Mucor hiemalis Wehmer; Phytate; Animal feed
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Purification and characterisation of an acid phosphatase with phytase activity from Mucor hiemalis Wehmer
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 132, Issue 1, 15 October 2007, Pages 82–87
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us