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Apolipoprotein E genotyping by multiplex tetra-primer amplification refractory mutation system PCR in single reaction tube

Paper ID Volume ID Publish Year Pages File Format Full-Text
25151 43556 2007 5 PDF Available
Title
Apolipoprotein E genotyping by multiplex tetra-primer amplification refractory mutation system PCR in single reaction tube
Abstract

Apolipoprotein E (APOE) plays a critical role in lipoprotein metabolism by binding to both low-density lipoprotein and APOE receptors. The APOE gene has three allelic forms, ɛ2, ɛ3, and ɛ4, which encode different isoforms of the APOE protein. In this study, we have developed a new genotyping method for APOE. Our multiplex tetra-primer amplification refractory mutation system (multiplex T-ARMS) polymerase chain reaction (PCR) was performed in a single reaction tube with six primers consisting of two common primers and two specific primers for each of two single nucleotide polymorphism (SNP) sites. We obtained definitive electropherograms that showed three (ɛ2/ɛ2, ɛ3/ɛ3, and ɛ4/ɛ4), four (ɛ2/ɛ3 and ɛ3/ɛ4), and five (ɛ2/ɛ4) amplicons by multiplex T-ARMS PCR in a single reaction tube. Multiplex T-ARMS PCR for APOE genotyping is a simple and accurate method that requires only a single PCR reaction, without any another treatments or expensive instrumentation, to simultaneously identify two sites of single nucleotide polymorphisms.

Keywords
Apolipoprotein E; Amplification refractory mutation system; Polymerase chain reaction; Tetra-primer; Single nucleotide polymorphism
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Apolipoprotein E genotyping by multiplex tetra-primer amplification refractory mutation system PCR in single reaction tube
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 131, Issue 2, 31 August 2007, Pages 106–110
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us