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Covalent attachment of cholesterol oxidase and horseradish peroxidase on perlite through silanization: Activity, stability and co-immobilization

Paper ID Volume ID Publish Year Pages File Format Full-Text
25152 43556 2007 10 PDF Available
Title
Covalent attachment of cholesterol oxidase and horseradish peroxidase on perlite through silanization: Activity, stability and co-immobilization
Abstract

In the present work, co-immobilization of cholesterol oxidase (COD) and horseradish peroxidase (POD) on perlite surface was attempted. The surface of perlite were activated by 3-aminopropyltriethoxysilane and covalently bonded with COD and POD via glutaraldehyde. Enzymes activities have been assayed by spectrophotometric technique. The stabilities of immobilized COD and POD to pH were higher than those of soluble enzymes and immobilization shifted optimum pH of enzymes to the lower pH. Heat inactivation studies showed improved thermostability of the immobilized COD for more than two times, but immobilized POD was less thermostable than soluble POD. Also activity recovery of immobilized COD was about 50% since for immobilized POD was 11%. The Km of immobilized enzymes was found slightly lower than that of soluble enzymes. Immobilized COD showed inhibition in its activity at high cholesterol concentration which was not reported for soluble COD before. Co-immobilized enzymes retained 65% of its initial activity after 20 consecutive reactor batch cycles.

Keywords
COD, cholesterol oxidase; POD, horseradish peroxidase; APTS, 3-aminopropyltriethoxysilane; 4-AAP, 4-aminoantipyrineCholesterol oxidase; Peroxidase; Co-immobilization; Perlite; Silanization; Thermostability
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Covalent attachment of cholesterol oxidase and horseradish peroxidase on perlite through silanization: Activity, stability and co-immobilization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 131, Issue 2, 31 August 2007, Pages 111–120
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us