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Testosterone 15β-hydroxylation by solvent tolerant Pseudomonas putida S12

Paper ID Volume ID Publish Year Pages File Format Full-Text
25164 43556 2007 4 PDF Available
Title
Testosterone 15β-hydroxylation by solvent tolerant Pseudomonas putida S12
Abstract

A steroid 15β-hydroxylating whole-cell solvent tolerant biocatalyst was constructed by expressing the Bacillus megaterium steroid hydroxylase CYP106A2 in the solvent tolerant Pseudomonas putida S12. Testosterone hydroxylation was improved by a factor 16 by co-expressing Fer, a putative Fe-S protein from Bacillus subtilis. In addition, the specificity for 15β-hydroxylation was improved by mutating threonine residue 248 of CYP106A2 into valine. These new insights provide the basis for an optimized whole-cell steroid-hydroxylating biocatalyst that can be applied with an organic solvent phase.

Keywords
CYP106A2; Whole cell biocatalyst; Steroid synthesis; Solvent tolerance; Pseudomonas putida; Fer
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Testosterone 15β-hydroxylation by solvent tolerant Pseudomonas putida S12
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 131, Issue 2, 31 August 2007, Pages 205–208
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us