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Activity and stability of alkaline phosphatase (ALP) immobilized onto magnetic nanoparticles (Fe3O4)

Paper ID Volume ID Publish Year Pages File Format Full-Text
25169 43558 2007 5 PDF Available
Title
Activity and stability of alkaline phosphatase (ALP) immobilized onto magnetic nanoparticles (Fe3O4)
Abstract

Direct binding of alkaline phosphatase (ALP) on magnetic nanoparticles (Fe3O4) in the presence of carbodiimide (CDI) using two different methods is described. The activity and stability of immobilized ALP with both shaking and sonication method were compared. The results indicated the ALP binding efficiency to be in the range of 80–100% with both the immobilization techniques. The activities retained were in the range of 20–38% with shaking method and 30–43% with sonication method, respectively. The activities of the immobilized ALP preparations were found to be stable compared to the free (unbound) ALP for at least 16-week storage period. Moreover, ALP immobilized on magnetic nanoparticles was successfully used for dephosphorylation of plasmid DNA before it was used for ligation reaction. The use of immobilized ALP for plasmid dephosphorylation allows easy manipulation, reduces the procedural time, and also avoids exposure of reaction mixture to high temperature.

Keywords
Plasmid dephosphorylation; Ligation; Protein binding; Carbodiimide
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Activity and stability of alkaline phosphatase (ALP) immobilized onto magnetic nanoparticles (Fe3O4)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 131, Issue 3, 15 September 2007, Pages 240–244
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
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Any Questions? feel free to contact us