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Pattern of anti-HIV dipyranocoumarin expression in callus cultures of Calophyllum inophyllum Linn.

Paper ID Volume ID Publish Year Pages File Format Full-Text
25186 43559 2007 8 PDF Available
Title
Pattern of anti-HIV dipyranocoumarin expression in callus cultures of Calophyllum inophyllum Linn.
Abstract

Callus cultures of Calophyllum inophyllum were established using seed, nodal/ internodal and leaf explants on WPM basal medium supplemented with indole-3-butyric acid (IBA), α-naphthalene acetic acid (NAA), picloram (4-amino-3,5,6-trichloropicolinic acid), and 6-benzylaminopurine (BAP) in different combinations and concentrations with the view to study the influence of hormones on callus induction and the pattern of expression of dipyranocoumarins including anti-HIV, non-nucleoside reverse transcriptase inhibitors inophyllum B and P in callus cultures. 96.01% seed explants, 87.50% nodal/internodal explants and 86.66% leaf explants were converted into calluses when inoculated on WPM supplemented with IBA 4.0 mg l−1 along with BAP 1.0 mg l−1, IBA 4.0 mg l−1, and picloram 6.0 mg l−1 along with BAP 2.0 mg l−1, respectively. Calluses induced from seed explants were white, friable and irregular whereas nodal/internodal and leaf explants induced dark brown, nodular and compact calluses. In order to facilitate the rapid quantitative analysis of dipyranocoumarins under study, a novel HPLC method capable of separating all six dipyranocoumarins in a single isocratic run has been optimized. Quantitative HPLC analysis of callus extracts revealed that highest inophyllum B (40.59 mg 100 g callus−1) was expressed in callus induced from seed explant on medium containing 2.0 mg l−1 indole-3-butyric acid, while highest inophyllum P (141.35 mg 100 g callus−1) was estimated in seed callus induced on medium containing 2.0 mg l−1 indole-3-butyric acid along with BAP 1.0 mg l−1.

Keywords
WPM, woody Plant Medium; IBA, indole-3-butyric acid; BAP, 6-benzyl amino purine; NAA, α-naphthalene acetic acid; PVP, poly vinyl pyrrolidone; HPLC, high performance liquid chromatography; NNRTIs, non-nucleoside reverse transcriptase inhibitors; RT, retent
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Pattern of anti-HIV dipyranocoumarin expression in callus cultures of Calophyllum inophyllum Linn.
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 130, Issue 4, 15 July 2007, Pages 346–353
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
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100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us