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An economical and highly productive cell-free protein synthesis system utilizing fructose-1,6-bisphosphate as an energy source

Paper ID Volume ID Publish Year Pages File Format Full-Text
25192 43559 2007 5 PDF Available
Title
An economical and highly productive cell-free protein synthesis system utilizing fructose-1,6-bisphosphate as an energy source
Abstract

In this study, we describe the development of a cost effective and highly productive cell-free protein synthesis system derived from Escherichia coli. Through the use of an optimal energy source and cell extract, approximately 1.3 mg/mL of protein was generated from a single batch reaction at greatly reduced reagent costs. Compared to previously reported systems, the described method yields approximately 14-fold higher productivity per unit reagent cost making this cell-free synthesis technique a promising alternative for more efficient protein production.

Keywords
Cell-free protein synthesis; Fructose-1,6-bisphosphate (FBP); Chloramphenicol acetyltransferase (CAT); S30 extract; S12 extract
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An economical and highly productive cell-free protein synthesis system utilizing fructose-1,6-bisphosphate as an energy source
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 130, Issue 4, 15 July 2007, Pages 389–393
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us