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Sugar-mediated lyoprotection of purified human CYP3A4 and CYP2D6

Paper ID Volume ID Publish Year Pages File Format Full-Text
25197 43559 2007 5 PDF Available
Title
Sugar-mediated lyoprotection of purified human CYP3A4 and CYP2D6
Abstract

P450 enzymes are of great interest for drug metabolism and as potential biocatalysts. Like most P450s, purified CYP3A4 is normally handled and stored in solution because lyophilization greatly reduces its activity. We show here that colyophilization of this enzyme with sucrose or trehalose, but not mannitol, crown ethers or cyclodextrins, allow recovery of full enzymatic activity after rehydration. Sorbitol was almost as efficient, with 85% retention of the original activity. We also show that similar protection is observed through colyophilization of CYP2D6 with trehalose. This procedure should greatly facilitate handling, storage, or use of these enzymes in anhydrous media.

Keywords
Biocatalyst; Cryoprotection; Freeze-drying; Lyophilization; P450; Saccharide
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Sugar-mediated lyoprotection of purified human CYP3A4 and CYP2D6
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 130, Issue 4, 15 July 2007, Pages 436–440
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us