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Application of the Cre-loxP system for multiple gene disruption in the yeast Kluyveromyces marxianus

Paper ID Volume ID Publish Year Pages File Format Full-Text
25205 43560 2007 7 PDF Available
Title
Application of the Cre-loxP system for multiple gene disruption in the yeast Kluyveromyces marxianus
Abstract

The yeast Kluyveromyces marxianus presents several interesting features that make this species a promising industrial yeast for the production of several compounds. In order to take full advantage of this yeast and its particular properties, proper tools for gene disruption and metabolic engineering are needed. The Cre-loxP system is a very versatile tool that allows for gene marker rescue, resulting in mutant strains free of exogenous selective markers, which is a very important aspect for industrial application. As the Cre-loxP system works in some non-conventional yeasts, namely Kluyveromyces lactis, we wished to know whether it also works in K. marxianus. Here, we report the validation of this system in K. marxianus CBS 6556, by disrupting two copies of the LAC4 gene, which encodes a β-galactosidase activity.

Keywords
Cre-loxP system; Kluyveromyces marxianus; Gene disruption; β-Galactosidase; Gene targeting efficiency
First Page Preview
Application of the Cre-loxP system for multiple gene disruption in the yeast Kluyveromyces marxianus
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 131, Issue 1, 1 August 2007, Pages 20–26
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering